abstract |
PROBLEM TO BE SOLVED: To provide a method for efficiently inducing proliferation in vitro and in vivo of neural stem cells which are most important for transplantation treatment of nerve damage or neurological dysfunction disease. SOLUTION: A mammalian neural tissue containing neural stem cells is isolated, and neural stem cells are selectively cultured in a culture medium containing a growth factor such as EGF or FGF, and then a neural stem cell and a surface marker of CD11c are formed on the cell surface. Co-cultured with dendritic cells such as immature dendritic cell subsets and blood cells such as spleen cells and CD8 positive T cells, or cultured neural stem cells in the presence of GM-CSF, Alternatively, the cultured neural stem cells are cultured in dendritic cell culture supernatant or blood cell culture supernatant. |