| abstract |
PROBLEM TO BE SOLVED: To provide a phagemid expression vector containing a transcriptional regulatory element operably linked to a gene fusion encoding a fusion protein. The gene fusion contains a first gene encoding a polypeptide and a second gene encoding at least a portion of a phage coat protein, and (a) the vector comprises a mature coat Does not contain the gene encoding the protein, (b) does not contain the complete phage genome, (c) transformation of the host cell with the vector does not produce phage particles in the absence of helper phage, or (d an amino acid-encoded triplet codon in which a DNA triplet codon encoding a repressible stop codon of mRNA is inserted between the fusion ends of the first and second genes or adjacent to the gene fusion junction A phagemid expression vector substituted for. [Selection figure] None |