http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005065575-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 2003-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de71d8f07c959db76da533c47b1176a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_424f4494d73e47ffa70469f2a2b35221 |
publicationDate | 2005-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2005065575-A |
titleOfInvention | Creation of protein molecular diversity population by frame shuffling |
abstract | PROBLEM TO BE SOLVED: To change random translation reading frames to DNA in which stop codons of three translation reading frames are excluded in advance in order to prepare an artificial protein gene library composed of mutants having large sequences separated from each other. To provide a method for producing a protein molecular diversity population by preparing a mutant population. A Y-family in which a gene DNA sequence in which a stop codon is excluded in advance is prepared from three types of translation reading frames of a natural gene DNA sequence, and the gene DNA sequence is frequently subjected to base mutation at the time of replication. By amplifying with a DNA polymerase such as DNA polymerase, a gene DNA population in which a frame shift is randomly introduced is prepared, and the gene DNA population is expressed by a conventional method to produce a protein molecule diversity population. [Selection] Figure 1 |
priorityDate | 2003-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 395.