| abstract |
Humanized rodent antibodies or fragments thereof, and humanized rodent monoclonal antibodies are provided. A three-dimensional model of the variable region of a rodent antibody is constructed, the conformational structure of the variable region of a rodent antibody or fragment thereof is determined, and X-rays are obtained for the heavy and light chains of the antibody variable region. By providing the position of a set of heavy and light chain frameworks from the accessibility distribution obtained from the crystal structure, determining the amino acid residues exposed on the surface, and surface reprocessing, the rodent antibody or How to determine how to humanize the fragment. [Effects] An easily manufactured rodent antibody can be applied to humans. [Selection] Figure 1 |