| abstract |
It is possible to easily and efficiently produce a fusion protein having α1,3-fucosyltransferase VI activity as a soluble protein in large quantities, and to easily obtain α1,3-fucosyltransferase VI itself from the fusion protein. Is possible. A recombinant fusion protein of a sugar-binding protein and α1,3-fucosyltransferase VI and a method for producing a recombinant fusion protein comprising the following steps (1) to (3). (1) Expression in which a DNA encoding a sugar-binding protein and a DNA encoding α1,3-fucosyltransferase VI are linked so as to be expressed as a fusion protein of both proteins under the control of a promoter capable of functioning in E. coli Using a vector, E. coli is transformed, (2) culturing the obtained transformant to produce a fusion protein of a sugar-binding protein and α1,3-fucosyltransferase VI; (3) Separating the fusion protein from the obtained culture [Selection] None |