| abstract |
An object of the present invention is to provide a colorimetric determination method with little influence of a reducing substance such as bilirubin in a sample, a method for stabilizing a measurement reagent, a method for suppressing the effect of bilirubin, and a long-term storage for the determination, using H 2 O 2 reagent. A colorimetric determination method in which creatinine, creatine, or uric acid in a sample is converted to hydrogen peroxide by an enzymatic reaction to produce a coloring dye in the presence of peroxidase. [Chemical 1] (R: H or methyl group) and formula (II): [Chemical 2] (R: H or methyl group; X: C12-20 alkyl) Colorimetric determination method, storage stabilization method, and bilirubin effect suppression method in which a polymer having a molecular weight of 5,000 to 5,000,000 consisting of units based on monomers is present, And a reagent containing the polymer. [Selection figure] None |