http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005000174-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_280d87031f0c5786de1808713a1b76b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3018de88a932b69d3caed9a8b8f64dad |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2004-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02d84a8cf0de963a1ee2eec8093f8ffa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3269e3e299e97de80c183606a9b1589 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5972a406b9578066c330db5ae2531e58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecd0c75570d897b806f689dfc0d33a80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29bf1b5690b72389ee4841608c7bb6c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6d8880a3762ad0a2c1856982d2ac578 |
| publicationDate | 2005-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2005000174-A |
| titleOfInvention | Method for determining the nucleotide sequence of oligonucleotides and DNA molecules |
| abstract | PROBLEM TO BE SOLVED: To provide a novel method for analyzing a nucleic acid sequence. Next steps: (a) providing a template system comprising at least one nucleic acid molecule of unknown sequence hybridized to a primer oligonucleotide in the presence of a DNA polymerase with low exonuclease activity; (b) allowing the template system and one deoxyribonucleotide to extend the primer by incorporating at least one deoxyribonucleotide having a fluorescent moiety into the 3 ′ end of the primer to form an extended primer Contacting under conditions, (c) detecting whether extension of the primer has occurred by detecting a fluorescent signal generated from the fluorescent component, and further destroying the fluorescent signal without removing the fluorescent component; (d) detecting the number of deoxyribonucleotides incorporated into the primer; (e) removing unincorporated deoxyribonucleotides; and (f) repeating steps (a) to (e) to determine the nucleotide sequence of the nucleic acid molecule; A DNA sequencing method comprising: [Selection figure] None |
| priorityDate | 1998-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 589.