| abstract |
To increase the yield of insoluble polypeptides in the form of inclusion bodies after recombinant expression. Expressing a nucleic acid encoding a polypeptide in a microbial host cell, forming an inclusion body containing the polypeptide in the cytoplasm of the host cell, and isolating, solubilizing, and naturalizing the polypeptide A process for recombinantly producing a desired polypeptide, wherein after fermentation, the host cells or host cell contents are incubated at a temperature of 40 ° C. or higher for at least 10 minutes, after which The peptide is isolated from the host cell. Provided is a method for recombinant production of a desired polypeptide that improves the yield of inclusion bodies containing the desired polypeptide. [Selection figure] None |