http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004537307-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate | 2002-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2004-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2004537307-A |
titleOfInvention | Methods and Supports for Biological Analysis Using Oligonucleotides Containing Enzyme-Activated Markers |
abstract | The present invention relates to a method for analyzing a DNA or RNA biological target, comprising the steps of: a) contacting the target to be analyzed (ICAM-20) with an oligonucleotide probe (ISIS) marked with cofactor A of enzyme E, b) reaction. A step of adding an enzyme E corresponding to cofactor A and a substrate S of the enzyme E to the medium, and converting the substrate S into a compound C by the enzyme E; c) a signal indicating the activity of the enzyme E with respect to the substrate S, for example, Measuring the fluorescence intensity of (I), and d) the signal is obtained by contacting the oligonucleotide probe labeled with cofactor A (ISIS) of enzyme E with the substrate S under the same conditions but without a target. Comparing to the signal obtained, a step in which the difference between the two signals indicates the presence of the complementary target (ICAM-20) of the oligonucleotide probe. |
priorityDate | 2001-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.