abstract |
The present invention is directed to detecting genetic variations in target nucleic acids, detecting the presence or absence of a particular nucleic acid in a biological sample, preparing a single-stranded nucleic acid probe, and targeting a target cDNA molecule or cDNA. Compounds, kits and methods for detecting pre-mRNA differential splicing in a population. It either contains a genetic variation of the target nucleic acid, has a unique sequence associated with a particular nucleic acid, is complementary to the nucleic acid of interest, or contains an exon-exon junction. Nick formation is used in the amplification of single-stranded nucleic acid fragments. |