http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004533259-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0034 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate | 2002-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004533259-A |
| titleOfInvention | Walkthrough technology for in vitro recombination of polynucleotide sequences |
| abstract | A simple and efficient method of random in vitro mutagenesis and recombination of polynucleotide sequences based on walkthrough with chain elongation and chain termination molecules such as dNTP / ddNTP, followed by reassembly and amplification is described. The utility of this method to improve protein structure and / or function is to create new sarcosine oxidase variants and to improve human variant placental alkaline phosphatase and calf intestinal alkaline for better mutants with improved properties Indicated by recombination of phosphatase. |
| priorityDate | 2001-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 245.