http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004520403-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-205 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2002-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004520403-A |
| titleOfInvention | Method for purifying Helicobacter adhesin-like protein A (ALPA) |
| abstract | The present invention is a method for purifying Helicobacter adhesin-like protein A (AlpA), comprising: (i) contacting an AlpA preparation and 2.5-3.5 M guanidine with a hydrophobic interaction chromatography material. And (ii) eluting AlpA with a solution containing 3.5-4.5M guanidine. The AlpA preparation to be purified can be obtained, in particular, from E. coli cultures having the ability to express AlpA in recombinants at high levels. Here, rAlpA is in the form of an inclusion body, which may be recovered, solubilized in the presence of guanidine, and precipitated with ammonium sulfate for pre-purification. After hydrophobic interaction chromatography, anion exchange chromatography can be performed in the presence of 8M urea. |
| priorityDate | 2001-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.