abstract |
The present invention relates to a method that can be used to detect mutations in different nucleotide sequences in parallel, wherein the method additionally comprises mutated and non-mutated nucleotides. It relates to the aforementioned method, which makes it possible to determine the transcription rate of the sequence. The method comprises hybridizing the single-stranded sample nucleotide sequence to a single-stranded reference nucleotide sequence, prior to or during the hybridization, to the single-stranded reference nucleotide sequence or the single-stranded sample nucleotide sequence, or to the hybridization. Immobilizing a heteroduplex consisting of the reference and sample nucleotide sequences on or after an electronically addressable surface, incubating with a substrate that recognizes the heteroduplex mismatch, after or during Detecting binding. |