http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004513617-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 |
| filingDate | 2001-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004513617-A |
| titleOfInvention | Methods and compositions for transcription-based nucleic acid amplification |
| abstract | A method for isothermal exponential amplification of a target polynucleotide is disclosed. This method uses two transfer modules. The first module provides for linear amplification resulting in RNA transcripts. And the second module provides further (generally cyclic) amplification that yields more RNA transcripts. In one aspect, amplification of the first module is based on composite primers. In a second aspect, amplification of the first module is based on target switching to generate a primer extension product containing a promoter sequence. In all aspects, the RNA transcript of the first transcription module is subjected to further amplification by creating an intermediate product comprising a double-stranded promoter region from which transcription can occur. The invention further provides compositions and kits for performing such methods, and methods of using the amplification results. |
| priorityDate | 2000-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 213.