http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004511233-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10343 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 |
| filingDate | 2001-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004511233-A |
| titleOfInvention | Method for circularizing adenovirus nucleic acids by homologous recombination |
| abstract | Applicants have identified a method that utilizes a bacterial homologous recombination system to convert a double-stranded linear adenovirus genome to circularized plasmid form (see FIG. 4). Such a system works with adenovirus terminal fragments present on the plasmid, each of less than 500 base pairs. The result is a plasmid that is more easily analyzed by restriction digestion, PCR, DNA sequencing or used in transient transfection studies. The resulting adenovirus plasmid can be rescued into a viral form. The entire procedure requires only four days or less, rather than the weeks required for plaque purification or diluted cloning isolation procedures. A further advantage of the present invention is that the disclosed method does not require the use of tissue culture materials or equipment. The disclosed method allows for a more extensive and thorough examination of virus samples in that it allows for the detection of variants that cannot grow without the help of a co-infected intact adenovirus genome. Under the standard conditions of plaque purification, these mutant genomes are not detected. It is expected that much more mutant genomes will be found using rapid methods than would be detected by standard plaque purification methods. |
| priorityDate | 2000-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.