abstract |
The present invention relates to methods and compositions for identifying modulators of β-secretase activity. More specifically, the present invention provides a novel substrate for monitoring the β-secretase activity of human Asp2 protease and a method using the same. Such methods and compositions are useful for identifying agents that modulate β-secretase activity, and thus can be used for therapeutic intervention in disorders characterized by the presence of amyloid plaques. Thus, the present invention provides peptide or fusion polypeptide substrates comprising a β-secretase cleavage site, and discloses methods and compositions for making and using the peptides. |