http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004500875-A
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-71 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2001-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2004-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2004500875-A |
titleOfInvention | Modified construct downstream of the initiation codon for overexpression of recombinant proteins |
abstract | The present invention relates to a construct for expression of a gene encoding a recombinant protein of interest placed under the control of the tryptophan operon Ptrp in a prokaryotic host cell, comprising the sequence of SEQ ID NO: 1 immediately downstream of its start codon. A nucleic acid sequence, and a multi-cloning cassette downstream of the sequence adapted to receive the gene encoding the recombinant protein of interest, wherein at least one of the nucleotides of SEQ ID NO: 1 is overexpressed of the recombinant protein. Mutated or deleted to allow for The invention also relates to a vector comprising such a construct, a prokaryotic host cell transformed with said vector, and a method for producing a recombinant protein of interest using the construct according to the invention. |
priorityDate | 2000-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 485.