http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004500850-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2001-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004500850-A |
| titleOfInvention | Nucleic acid detection substance and detection method |
| abstract | An analysis using an unnatural base is described. In one embodiment, the method contacts a sample suspected of containing a target nucleic acid with a nucleic acid polymerase and first and second primers; if the target nucleic acid is present in the sample, the first and first Amplify the target nucleic acid by PCR using two primers to produce an amplification product having a double stranded region and a single stranded region containing an unnatural base; A sample is contacted with a reporter containing a non-natural base; at least a portion of the reporter is annealed to a single-stranded region of the amplification product; after annealing, at least a portion of the reporter is cleaved to obtain at least one reporter fragment And correlating the release of at least one reporter fragment with the presence of the target nucleic acid in the sample. The present invention also provides a corresponding kit for use in detecting a target nucleic acid in a sample. Alternatively, the reporter can be incorporated into the amplification product rather than annealed and then cleaved. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2006095550-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016127825-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013507971-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006095550-A1 |
| priorityDate | 2000-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 500.