http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004321127-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d0f52bb4069f8a19d70dcfb697c67a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2003-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c38f1c43e7f20e1500001aab5b96a2d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72d3950485783bd8d67269f063e29773 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38f6ecd31ba342824140c2b6d2975b7b |
| publicationDate | 2004-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004321127-A |
| titleOfInvention | Method for detecting target nucleic acid fragment |
| abstract | The present invention provides a method for quantitatively measuring the amount of nucleic acid generated in association with a polymerase reaction using pyrophosphate generated in association with the polymerase reaction. (1) A target nucleic acid fragment having a known base sequence, at least one primer complementary to a part of the target nucleic acid fragment, at least one deoxynucleoside triphosphate, and at least one polymerase. And a step of performing a polymerase extension reaction starting from the 3 ′ end of the primer using the target nucleic acid fragment as a template, using a reaction mixture containing a divalent cation necessary for the polymerase reaction; and (2) A method of detecting whether or not the polymerase extension reaction proceeds continuously by detecting pyrophosphate generated in association with the polymerase extension reaction; Polymerer is the first reaction to detect the accompanying pyrophosphate The detection method described above, wherein the detection method is carried out simultaneously with a ZE extension reaction. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102758003-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006217909-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005093088-A1 |
| priorityDate | 2003-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 367.