abstract |
A method for easily detecting phosphorylation of a protein kinase substrate immobilized on a chip is provided. A method for detecting phosphorylation of an at least one peptide serving as a substrate of a protein kinase on an array having at least one peptide immobilized on a metal thin film of a substrate, comprising: A method for analyzing protein kinase activity, wherein a chelate compound, particularly a compound represented by the formula (I), is allowed to act upon detection, and the interaction is analyzed by surface plasmon resonance (SPR). Embedded image [Selection diagram] None |