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filingDate 2003-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d30cedb897911102755155a12e3708c
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publicationDate 2004-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2004301715-A
titleOfInvention Protein primary structure information collection method, hydrophobic fine particle carrier used therefor, and protein primary structure information automatic acquisition system
abstract Kind Code: A1 A method for collecting primary structure information of a protein, including a method of fragmenting a protein, which enables the automatic acquisition of primary structure information from a protein sample, an apparatus used for the method, and automatic analysis of primary structure information of the protein sample. Provide system. The method includes denaturing, reducing, and alkylating a protein, immobilizing the obtained reduced alkylated protein on a hydrophobic fine particle carrier, and chemically or enzymatically converting the obtained immobilized protein. And a step of obtaining primary structure information of the fragmented peptide. Analyze data obtained by the automatic fragmentation unit for automatically fragmenting reduced alkylated proteins, the automatic mass spectrometry unit for automatically performing mass spectrometry of fragmented peptides, and the automatic mass spectrometry unit For automatically obtaining primary structure information of a protein including a data analysis unit for performing the automatic fragmentation of the reduced alkylated protein using a hydrophobic fine particle carrier. [Selection diagram] Fig. 1
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