| abstract |
An object of the present invention is to provide a method for producing mannosylerythritol lipid, which can improve the production efficiency (production amount, production rate, and yield) of mannosylerythritol lipid, which is a kind of biosurfactant. A method for producing mannosylerythritol lipid by culturing a microorganism having the ability to produce mannosylerythritol lipid in a culture solution containing vegetable oil and nutrients, wherein the pH of the culture solution is 5.2 to 5.8. A method for producing mannosyl erythritol lipid, wherein the culture is performed while controlling the culture within the range described above. An embodiment in which the initial vegetable oil concentration is in the range of 10 to 20% by mass, the culture is started in the initial vegetable oil concentration of 14 to 20% by mass, and the plant oil is reduced to 0 to 6 days after the start of the culture. An embodiment in which the total vegetable oil concentration, which is the sum of the initial vegetable oil amount and the fed-batch vegetable oil amount, is 25% by mass or more. It is preferable to perform the operation. [Selection diagram] None |