abstract |
For a sample that does not reach a certain threshold value within a measurement time, the time to reach a certain threshold value without extending the measurement time can be determined by using data of a change in transmitted light or absorbance obtained within the measurement time. An apparatus and method are provided for estimating and enabling sensitive measurements. The ratio of the amount of transmitted light or absorbance after a test sample is mixed with a gelling or dye-forming reagent but before the change by the reaction to the amount of transmitted light or absorbance that changes over time is measured, Alternatively, the change over time (time course) of the ratio due to the reaction of the test sample is monitored, and the time until the ratio reaches a predetermined constant value (threshold for determination of gelation or dye formation) is measured, or If the gelation or dye generation determination threshold has not been reached at the end of the measurement time, the monitored time course is extended to the time after the measurement time by the estimation calculation, and the estimated time course curve is calculated. By calculating the time until the gelation or dye development determination threshold is reached, the short-time measurement of a sample that does not reach a certain threshold within the measurement time Possible and the. [Selection diagram] Fig. 4 |