| abstract |
An object of the present invention is to provide a method for testing the activity of a substance that may be effective in inhibiting the enzyme activity of phospholipase A2. A) Potentially effective substances are -phospholipase A2, and at least one fatty acid in the form of -esters, preferably long-chain fatty acids having 15 to 22 carbon atoms, more preferably Is a substrate that is a phospholipid containing an unsaturated or polyunsaturated fatty acid, wherein the substrate is capable of releasing at least one fatty acid upon hydrolysis thereof; and b) In particular, a method comprising measuring the enzymatic activity of said phospholipase A2, comprising detecting the presence of said fatty acid and optionally determining its amount. This test method makes it possible in particular to identify and select active ingredients capable of inhibiting the enzymatic activity of phospholipase A2. [Selection diagram] Fig. 1 |