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filingDate 2003-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_803c2286078c59647a8a3d4cc95c0e93
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publicationDate 2004-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2004157119-A
titleOfInvention Pretreatment method for immunoassay for 8-isoprostane
abstract PROBLEM TO BE SOLVED: To provide a pretreatment method for measuring 8-isoprostane by immunoassay, which method enables more accurate immunoassay of 8-isoprostane than a known method. SOLUTION: (1) a step of applying a deproteinized and defatted sample to an NH2 column, (2) washing the column with a 5-20 v / v% 2-propanol solution, (3) washing the column with a 20-30 v / v% 2-propanol solution, (4) a step of elution with a 25-70 v / v% 2-propanol solution, (However, the concentration of 2-propanol in the solution of (2) to (4) is (2) <(3) <(4)) in this order. The solution eluted in the elution step (4) is subjected to immunoassay to measure the concentration of 8-isoprostane. [Selection diagram] None
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012194177-A
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priorityDate 2002-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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