| abstract |
The present invention provides an immortalized NK cell line that retains the original functions and characteristics of NK cells, a method for establishing the immortalized NK cell line, a screening method for useful substances using the immortalized NK cell line, and a cell vaccine. Natural killer cells obtained by isolation from the spleen of a transgenic mouse introduced with a large T antigen gene of SV40 temperature-sensitive mutant tsA58 are cultured to have azurophilic granules in the cytoplasm. A cell line is established which retains the ability to kill target cells and / or the ability to kill target cells coated with an antibody without any growth, and which proliferates and activates with interleukin-2. |