http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004141158-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be055db3c1a09879df07379ba969e223 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
| filingDate | 2003-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07c8fdf6531fd24e4723fe10016474b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f84682c1ecb7c5c4720012c556284672 |
| publicationDate | 2004-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004141158-A |
| titleOfInvention | Primer extension reaction detection method, base type determination method, base type determination device, pyrophosphate detection device, nucleic acid detection method, and sample solution introduction chip |
| abstract | An object of the present invention is to provide a simple technique for determining a base type in a base sequence of a nucleic acid. A step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence including a complementary binding region that complementarily binds to the nucleic acid, and a nucleotide, the extension reaction of the sample solution with the primer (B) generating pyrophosphoric acid when the elongation reaction occurs under the conditions where H + -pyrophosphatase penetrates the front and back, and has an active site for hydrolyzing pyrophosphate exposed on the surface. contacting the sample solution to the surface of the H + hardly permeable membrane having (c), the H + - in a state of being immersed pyrophosphatase solution, the surface of the H + hardly permeable membrane solution or the H + step of measuring at least one of H + concentration of the back surface side of a solution of poorly permeable membrane (d), the step step of detecting the extension reaction on the basis of the measurement results of (d) (e , And steps on the basis of the detection result (e) comprises a step (f) to determine the base type of the base sequence of the nucleic acid. [Selection diagram] FIG. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10822641-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013116081-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8753812-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012283107-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9228971-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7309574-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005003750-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2888374-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7291472-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013084917-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7285387-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005093088-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108865647-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021198533-A1 |
| priorityDate | 2002-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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