http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004113145-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d0f52bb4069f8a19d70dcfb697c67a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
| filingDate | 2002-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a50574defb034ab62522cd104e06605f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3952ba54bbe5f91c3d2d01be708f4c79 |
| publicationDate | 2004-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2004113145-A |
| titleOfInvention | Optical detection of multi-stranded nucleic acids |
| abstract | The present invention provides a method capable of detecting a multi-stranded nucleic acid with high sensitivity and ease in a neutral to weakly basic pH region used for biochemical examination. A method for optically detecting a multi-stranded nucleic acid by contacting a multi-stranded nucleic acid with a compound that emits substantial fluorescence by interaction with the multi-stranded nucleic acid, wherein the compound is represented by the following general formula (I): ) To (IV) (R 1 to R 3 are hydrogen atoms or substituents; R 4 is an alkyl group, etc .; Q is an oxygen atom or sulfur atom; n is an integer of 0 to 3; X is a counter ion; The number necessary to neutralize the charge; at least one of R 5 , R 7 , R 10 , and R 12 is an electron-donating substituent having a Hammett σp value of −1.5 or more and less than 0; R 6 , R 8 , R 9 , and R 11 represent a hydrogen atom or a monovalent substituent), and (a) substantially in aqueous solution under the condition of protonation in the absence of multi-stranded nucleic acid In a colorless and non-fluorescent state, (b) above (a) The method is a compound substantially changed to colored by interaction with the multi-stranded nucleic acid, and expressing substantially fluorescent by the interaction when the presence of multi-stranded nucleic acid under conditions. [Chemical 1] |
| priorityDate | 2002-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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