abstract |
An object of the present invention is to develop a labeled bacterial monitoring method using chitinase and chitosanase genes, which are chitinolytic enzymes, as reporters. The present invention relates to a method for detecting a bacterium into which a chitinolytic enzyme gene has been introduced, Contacting the bacterium into which the chitinolytic enzyme gene has been introduced with a bacteriophage specific to the bacterium; Reacting a lysate of the bacterium lysed by infection with the bacteriophage and a chitinolytic enzyme substrate, Detecting the degradation of the substrate; A method comprising: [Selection] Figure 2 |