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filingDate 2002-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_448421ed08c1588e5102405ab794d29a
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publicationDate 2004-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2004081042-A
titleOfInvention Method for preparing protein solution, said protein solution, method for producing monoclonal antibody using said protein solution, and said monoclonal antibody
abstract Kind Code: A1 A method for preparing a protein solution capable of dissolving a protein derived from an intracellular organelle and retaining a structure considered to be close to a natural structure in a solvent such as water at a high concentration, and the protein It is intended to provide a solution. (A) contacting an intracellular organelle with an organic acid solution to extract a protein contained in the intracellular organelle to prepare a protein organic acid solution; and (b) preparing the protein organic acid solution. Lyophilizing to prepare a protein powder, (c) dissolving the protein powder in a denaturant solution to prepare a protein denaturant solution, and (d) dissolving the protein denaturant solution in water or phosphate buffer. A method for preparing a protein solution in which various proteins contained in intracellular organelles are dissolved, comprising a step of preparing a protein solution by dialysis, and the protein solution. [Selection diagram] Fig. 1
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