| abstract |
PROBLEM TO BE SOLVED: To provide a method for determining whether an enveloped minus-strand single-stranded RNA virus having a genome encoding a large protein L of HPIV-3 is attenuated. A method for determining whether an enveloped negative-stranded single-stranded RNA virus having a genome encoding a large protein L of HPIV-3 has been attenuated, comprising: a wild-type HPIV-3 ( JS) ascertaining the presence of at least one substitution in the genome of the virus, as compared to the genome of JS), wherein the substitution is in a region of the genome encoding the L protein, A method characterized in that it is selected from the group consisting of alternative His, Phe instead of Leu at residue 992, and Ile instead of Thr at residue 1558. [Selection diagram] None |