| abstract |
(57) Abstract: Liposomes having a different lipid composition between the inner and outer bilayers and capable of reaching the primary tumor and its metastases after intravenous injection into animals and humans Methods for encapsulating a plasmid, oligonucleotide, or negatively charged drug are disclosed. This procedure comprises a fusogenic / NLS consisting of a DNA containing cationic lipid molecules and a hydrophobic chain of about 10-20 amino acids, and also containing at least one histidine residue or NLS at one of their ends. It involves complex formation with the peptide conjugate. The encapsulated molecules have a therapeutic effect in eradicating various solid human tumors, including but not limited to breast and prostate cancer. Liposomes encapsulating plasmids, oligonucleotides, or combinations of negatively charged drugs with other anti-neoplastic agents (positively charged cisplatin, doxorubicin) have therapeutic value. |