| abstract |
(57) [Summary]nThe present invention relates to transgenic animals, and to methods and compositions relating to characterizing gene function, and more particularly to genes encoding trinucleotide repeat proteins (TRP) such as gene T243. The invention provides a cell, preferably a stem cell, more preferably an embryonic stem (ES) cell, comprising a disruption in the target DNA sequence encoding TRP. The disruption is created by obtaining a sequence homologous to the target DNA sequence and inserting that sequence into a targeting construct. The targeting construct is then introduced into stem cells, resulting in homologous recombination that results in disruption of the target DNA sequence. |