http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2003526344-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2001-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2003-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2003526344-A |
titleOfInvention | Pyrophosphate degradation activated polymerization (PAP): application to allyl-specific amplification and nucleic acid sequencing |
abstract | (57) [Abstract] A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are sequentially linked for each amplification by using an activatable oligonucleotide P * with a non-extendable 3'-deoxynucleotide at the 3 'end. . PAP can be applied to exponential or linear amplification. PAP is one or more by using an activatable oligonucleotide P * that matches exactly at the 3 'end for rare alleles, but has a mismatch at or near the 3' end for wild-type alleles. It can be applied to the amplification of rare alleles in a mixture having the above wild-type alleles. PAP is inhibited by a mismatch in the 3 'specific subsequence up to 16 nucleotides from the 3' end. PAP can greatly increase the specificity of detecting very rare mutant alleles in the presence of wild-type alleles. Specificity arises from pyrophosphorolysis and polymerization, since significant nonspecific amplification requires a combination of mismatched pyrophosphorolysis and incorrect integration by DNA polymerase, a very rare event. It is. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011504732-A |
priorityDate | 2000-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 183.