| abstract |
(57) [Summary] The molecular recognition of cDNA encoding human serine protease, which we call a protease, will be described. The deduced amino acid sequence, and alignment with other well-characterized serine proteases, indicates that it is a member of the S1 serine protease family. We have found that protease CE mRNA is expressed in certain regions of the brain, namely the cerebellum, cerebral cortex, pituitary gland and hippocampus, as well as in the pancreas, placenta, small intestine, stomach, spleen, fibroblasts and epidermis. I discovered that. Enzymatically active proteases, as produced using the methods described herein, are amenable to further biochemical analysis for the identification of physiological substrates and specificity modulators. |