| abstract |
(57) SUMMARY The present invention provides attenuated vaccines and methods of obtaining attenuated vaccines. The vaccines of the present invention include recombinant viruses, bacteria, parasites, and other organisms that have been evolved to exhibit increased virulence attenuation without losing effectiveness as a vaccine. The method of the present invention involves the creation of a library of recombinant nucleic acids (eg, a whole or partial genome, or a specific nucleic acid), which library is introduced into a vaccine virus or other organism, and then Screening and / or selecting those viruses or organisms to be attenuated. |