| abstract |
(57) [Summary]nThe present invention relates generally to compositions, kits, and methods for labeling nucleic acid molecules using a reverse transcriptase, preferably a multi-subunit reverse transcriptase (eg, ASLV reverse transcriptase). . In particular, the present invention relates to methods, kits, and compositions for fluorescently labeling a nucleic acid molecule during nucleic acid synthesis with a fluorescent molecule or marker selected from the group consisting of rhodamine, fluorescein, Cy3, and Cy5. . Labeled nucleic acid molecules produced according to the present invention are particularly suitable as labeled probes for nucleic acid detection and diagnostics. |