http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2003500013-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 |
filingDate | 2000-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2003-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2003500013-A |
titleOfInvention | Cleavage of nucleic acids from solid supports |
abstract | (57) Abstract: The present invention relates to a method comprising the step of selectively cleaving a nucleic acid molecule at a specific nucleotide site at a specific site in the nucleic acid molecule, the method comprising: A method is provided for separating the nucleic acid molecule from a solid support to which the molecule is attached. The nucleic acid molecule may be a chimeric molecule comprising the nucleic acid (nucleotide sequence) and other molecular components having different chemical properties. According to the present invention, there is provided a chimeric molecule (construct) comprising a nucleotide linker sequence having a specific nucleotide capable of selective cleavage, which is bound to a functional group which may be an affinity binding group or a reporter group at a predetermined position. You. The invention is useful in a number of applications, including molecular biological manipulations such as nucleic acid synthesis, amplification, sequencing, nucleic acid separation, affinity-based separation and assay manipulation. |
priorityDate | 1999-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 497.