| abstract |
(57) [Summary] [Problem] A surrogate substance capable of performing a trace analysis of a sulfate conjugate or a glucuronide conjugate of estradiol with high accuracy and high reliability, and using the surrogate substance. And a method for microanalyzing an estradiol conjugate. SOLUTION: At least one of the hydroxyl groups at the 3-position and the 17-position is protected with glucuronic acid or sulfuric acid or a salt thereof, and the remaining hydroxyl groups may be salts, and at least one carbon atom in the molecule. Is labeled with a stable isotope, and a method for microanalyzing an estradiol glucuronic acid conjugate or an estradiol sulfate conjugate, wherein the estradiol conjugate is used as a surrogate substance. |