| abstract |
(57) [Summary] (with correction) [PROBLEMS] To provide a method for easily obtaining a high ratio of myeloid cells to erythroid cells (M / E ratio). SOLUTION: (1) A bone marrow fluid sample is divided into two samples, (2) One sample is mixed with a first hemolytic agent to lyse erythrocytes, mixed with a first staining solution containing a fluorescent dye and stained, and the other sample is mixed with a second hemolytic agent for myeloid immature cells. Other cells are damaged, mixed with a second staining solution containing a fluorescent dye, and stained. (3) Each sample stained in (2) is introduced into a flow cytometer, and scattered light and fluorescence are measured. 4) Step (2) Using the difference in intensity between the scattered light and the fluorescence of the sample stained with the first staining solution, the white blood cells, erythroid cells and lipid particles are classified and counted, and the sample stained with the second staining solution in step (2) The myeloid mature leukocytes, lymphoid leukocytes and myeloid immature cells are classified and counted using the scattered light and fluorescence of ) The M / E ratio is calculated from the number of erythroid cells and the number of myeloid cells. |