| abstract |
PROBLEM TO BE SOLVED: To provide a DNA sequence and a method enabling production of plants, fungi and microorganisms capable of synthesizing linear α-1,4 glucan. SOLUTION: The present invention provides a protein having an amylosucrase enzymatic activity capable of synthesizing a linear α-1,4 glucan from a substrate sucrose by bacteria, fungi and plants, or in a cell-free system. Related to the DNA sequence to be encoded. The present invention further discloses a plasmid and a bacterium containing the DNA sequence, and a method for producing a plant and a microorganism capable of expressing a polypeptide having amylosucrase activity inside or outside a cell. The present invention further relates to a method for producing pure fructose using a protein exhibiting amylosucrase enzymatic activity. |