| abstract |
(57) [Summary] (Problem corrected) [Problem] To provide a glucose dehydrogenase having heat resistance, alkali resistance and / or improved specific activity which has not been known hitherto by genetic engineering techniques, and recombining the same. Provided are a vector, a transformant, a method for producing glucose dehydrogenase using the transformant, and a glucose assay kit. The present invention relates to a glucose dehydrogenase containing a specific amino acid sequence, a glucose dehydrogenase containing one or more amino acid sequences deleted, substituted or added, and having a heat resistance in the absence of an inorganic salt. Dehydrogenases having improved sex, alkali resistance and / or improved specific activity, genes encoding these glucose dehydrogenases, genes containing specific nucleotide sequences, recombinant vectors containing these genes, transformants And a method for producing glucose dehydrogenase, comprising collecting glucose dehydrogenase from a culture of the transformant, and a glucose assay kit. |