Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2002-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6e8ff8d3bf814733f8d1e60ad1eb8f5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62bbf97bce7d0f0f7c1c47a4f4631ca5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faa2dd5e79ef45b7880feccf81aa6c34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24bcca0733504c6bdf6fc0bba4e5583e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fcab6c4c53957755f48d399b89960c4 |
publicationDate |
2003-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2003230390-A |
titleOfInvention |
E. FIG. Method for cloning and expressing BSMBI restriction endonuclease and BSMBI methylase in COLI, and method for purifying BSMBI endonuclease |
abstract |
The present invention relates to a recombinant DNA encoding a BsmBI restriction endonuclease and a BsmBI methyltransferase, and to the expression of a BsmBI restriction endonuclease and a BsmBI methylase in Escherichia coli cells containing the recombinant DNA. The present invention also provides It also relates to a method for purifying a recombinant BsmBI restriction endonuclease. The present invention relates to an isolated DNA encoding a BsmBI restriction endonuclease, comprising Bacillus stearothermophilus (Bacillus). isolated DNA available from B. stearothermophilus B61 and Bs of Bacillus stearothermophilus B61 by methylase selection and inverse PCR. Methods for cloning mBI restriction endonuclease into E. coli are provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002313965-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4637397-B2 |
priorityDate |
2001-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |