| abstract |
PROBLEM TO BE SOLVED: To provide a simple and highly sensitive method for measuring a compound producing formaldehyde as a reaction intermediate using glutathione-dependent formaldehyde dehydrogenase, And a reagent composition therefor. SOLUTION: Alcohol oxidase is allowed to act on a sample, and formaldehyde generated by the enzyme reaction is reacted with Alternatively, uricase is allowed to act on the sample, and hydrogen peroxide generated by the enzymatic reaction is further allowed to act on glutathione and glutathione-dependent formaldehyde dehydrogenase on formaldehyde generated by reacting with catalase in the presence of methanol. A method for measuring uric acid, comprising analyzing a compound produced from the enzyme reaction. |