Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_915269922949d81a90260e5cd5d2df40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc45703f67bc72a6db582867886fd5d5 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-85 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2002-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88c9585002266c26a03cf8edcf4dc19c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc986d9e500b778c8f5a40a3b14ed80a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2150ab496ac143b0c2892686786a7d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56e1b5def5199bb80a3e529a7e72fbaa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf46a708648f9681dcb327bac44b171e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b362c73579b477d0d17618ea00863020 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_916f838fb966f4d91da33b18e00dc7c4 |
publicationDate |
2003-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2003164295-A |
titleOfInvention |
Gene high expression system |
abstract |
(57) [Summary] (Modifications) [PROBLEMS] To provide a method for stably introducing a target gene into a host organism and highly expressing the gene. A pyruvate decarboxylase 1 promoter in the yeast Saccharomyces cerevisiae is linked to a gene of interest to be expressed and introduced into the genome of a host to stably and highly express the gene of interest. I found it. That is, a gene fermentation method characterized by introducing a target gene into a genome under the control of a promoter of a gene having an autoregulation mechanism or a promoter of a gene not essential for growth or fermentation in a host organism. It may be a DNA containing a sequence in which 1 to 40 bases have been deleted, substituted or added in the base sequence of the promoter, and which has promoter activity. Alternatively, a DNA having a promoter activity that hybridizes under stringent conditions to a DNA consisting of a sequence complementary to all or part of the base sequence of the above promoter may be used. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7964382-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006288318-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009034036-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4700395-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7696333-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005045024-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006296377-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009017233-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2004288072-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006006271-A |
priorityDate |
2001-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |