abstract |
(57) Abstract: A cell-based assay and sequence for PDE10. The present invention relates to a method of screening for an agent that inhibits intracellular phosphodiesterase 10A activity, the method comprising using the agent as a medium striatal spiny. Administering to neurons and submaximally activating adenylate cyclase, administering the agent to striatal medium spiny neurons, and activating guanylate cyclase to submaximal, and cAMP in cells Production and cGMP production were measured and cAMP E Including calculating C 200 and cGMP EC 200 , If the ratio of cAMP EC 200 / cGMPEC 200 is comparable to that produced by administration of papaverine under the same assay conditions, the agent is identified as a PDE10A inhibitor. It features the method. Also featured are rat PDE10A polynucleotide and polypeptide sequences. |