http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2003070497-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9779afa74a3c3f6ff93275907bfe44f3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72f5c90532d4b9691b256239bab6793c |
| publicationDate | 2003-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2003070497-A |
| titleOfInvention | Method for detecting nucleic acid deletion sequence |
| abstract | (57) Abstract: A method for determining the presence of a deleted portion in a nucleic acid is provided. In the method of the present invention, a sample suspected of containing the nucleic acid of interest is contacted in short PCR with reagents containing appropriate reagents and primers adjacent to the deletion sequence. Nucleic acids in contact with these substances are amplified and confirmed. Wild-type nucleic acids having a relatively long sequence between each sequence that hybridizes to the primer are not amplified. On the other hand, the mutant nucleic acid is amplified. Thus, detection of the amplicon provides a signal indicating the presence of a nucleic acid sequence having a deleted portion. By contacting this sample with a cleavage reagent specific for the deletion sequence, the wild-type DNA is cleaved, but the mutant nucleic acid not containing the deletion sequence is not cleaved. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012521745-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023058522-A1 |
| priorityDate | 2001-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.