| abstract |
[PROBLEMS] To provide a Schwann cell culture method capable of efficiently obtaining a large amount of Schwann cells. SOLUTION: 5-15% of peripheral nerve pieces treated with an enzyme After culturing in FCS-containing BS medium for 0-60 hours, culturing in serum-free BS medium for 12-60 hours to induce migration of Schwann cells under inhibition of fibroblast proliferation, then 1-10% FCS The cells are cultured in the BS medium for 7 days or longer, and Schwann cells are grown under the growth control of fibroblasts. In addition, after culturing in the above serum-free BS medium, peripheral nerve pieces taken out of the system were treated with serum-free BS medium and FCS. Schwann cells can also be grown by sequentially culturing in the BS medium containing. Furthermore, when Schwann cells are cultured on a bioabsorbable sheet, a cultured Schwann cell regeneration material is obtained. |