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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
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filingDate 2001-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3705263791d1bb299655cff76b68af10
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publicationDate 2003-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2003052395-A
titleOfInvention Transplant adequacy determination method
abstract (57) [Summary] [Problem] It is possible to easily determine whether transplantation is appropriate. SOLUTION: The transplantation suitability judging method of the present invention is a method wherein a cultured tissue after culturing is used as a measurement target in a tissue state without performing dispersion treatment, and an intracellular enzyme in the cultured tissue to be measured is reacted. Treats itself with an enzyme activity measurement reagent that is converted to a spectroscopically measurable substance, spectrophotometrically quantifies the converted substance, and determines the transplantation suitability of the cultured tissue based on the quantified result. Is what you do. In this transplantation suitability determination method, since the cultured tissue after culturing is used as a measurement target in a tissue state without performing dispersion treatment, the measurement target is not damaged by the dispersion treatment. In addition, the activity of the intracellular enzyme in the cultured tissue is quantified using an enzyme activity measurement reagent, but since this enzyme activity is correlated with the number of viable cells, the viable cell rate can be obtained as a numerical value as a result, There is no need to count the number of cells under a microscope.
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