http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2003052373-A

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a112ebf9fb16c633f45f1d037802a14d
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00
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filingDate 2001-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_679d4f0452d02ee38a3a715b971ac324
publicationDate 2003-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2003052373-A
titleOfInvention Sensor chip and base sequence detection method using calcium ion-dependent short-chain deoxyribozyme
abstract (57) [Summary] [Problem] To pay attention to the fact that the expression of ribozyme catalytic activity consisting of a specific sequence depends on calcium ions, for example, DNA DNA or RNA containing specific base sequence on microarray Is immobilized and detected by two methods: detection of gene sequence (RNA) fragments generated by a catalytic reaction in the presence of calcium ions and detection by complementary binding in the absence of calcium ions (conventional method). The present invention also provides a sensor chip and a base sequence detection method using a calcium-dependent short-chain deoxyribozyme having higher analysis accuracy than conventional methods. A specific sequence structure (dGGCTACAACG) is provided on the surface of a substrate. A sensor chip to which a short-chain deoxyribozyme having the nucleotide sequence of A) is bound.
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